LSTrAP-denovo is an easy-to-use pipeline to assemble and annotate transcripts for species without genome / genomic annotations using publicly available RNA-seq data.

De-novo Transcriptomics Assembly workflow for four Dictyostelium species (e.g.- Dictyostelium discoideum, Polysphondylium pallidum, Dictyostelium Lacteum and Dictyostelium Fasciculatum). This is the standard assembly workflow that should ideally work on any organism.Before normalizing first concatenate all RNAseq data across all samples into a s…