Mapping single-cell-resolution cell phylogeny reveals cell population dynamics during organ developments
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Single Molecule Sequecing Pipeline (smrt)
- Adaptors Trimming
- Circular Consensus Sequence Generation
- Align to Reference Sequence
- Annotate Sequence Features
- Link Sample Barcode Sequence
- Cluster Molecular Indentifier (UMI) Sequence
- Group Reads by Sample Barcode and Molecular Indentifier
- Generate Consensus Sequence for Lineage Barcode
- Call Mutation
- Post Quality Filter
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Single Cell Tree Construction (tree)
- Build Tree by Maximum Likelihood Method
- Fix Tree
- Visualize Tree
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Benchmarking Cell Lineage Tracing System (benchmark)
alemany_whole-organism_2018
bowling_engineered_2020
chan_molecular_2019
chen_efficient_2020
kalhor_developmental_2018
lee-six_population_2018
mckenna_whole-organism_2016
pei_polylox_2017
quinn_single-cell_2021
raj_simultaneous_2018
spanjaard_simultaneous_2018
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Simulation (simu)
- Generate Cell Tree (division / differentiation / mutation)
- Subsample Barcoded Cells
- Reconstruct Cell Lineage Tree
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Population Dynamics (pop)
- ?
scientific question : https://celllineage.github.io/SLOTH/index.html
technical details: https://github.com/CellLineage/SLOTH/wiki
git clone https://github.com/CellLineage/SLOTH.git
Liu, K., Deng, S., Ye, C. et al. Mapping single-cell-resolution cell phylogeny reveals cell population dynamics during organ development. Nat Methods 18, 1506–1514 (2021). https://doi.org/10.1038/s41592-021-01325-x
The content of this project itself is created by Ye Chang and licensed under the Creative Commons Attribution 4.0 International License (CC BY), and the underlying source code used to format and display that content is licensed under the MIT license.