Skip to content

A short macro to generate an image with randomly positioned "fluorescent" single molecules

License

Notifications You must be signed in to change notification settings

pmascalchi/ImageJ_SingleMoleculeSimulatedImage

Repository files navigation


Single Molecule image simulator_1.0

Summary:

This macro creates an image with one or several single molecules randomly positioned. Signal is built from a gaussian-fluctuating background plus 2D gaussian shapes representing individual molecules. Amplitude of the gaussian curve is randomized with user-defined min and max. Other user-defined parameters are: size of the image, nummber of molecules, background mean intensity and noise standard deviation. XY coordinates and gaussian amplitude are printed in a log table (comma-separated format).

Methods:

  1. Drag & drop the macro file on menu window

  2. Edit the parameters in the macro text

  3. Click “Run”


Releases

No releases published

Packages

No packages published