Rd0002

Tellurite resistance- and adherence-conferring island

Location

sequence            start    end      direction
TY-2482_chromosome  5252587  5272686  1
TY-2482_chromosome  1        65686    1

scaffold00001       583920   675596   1   

Attachment site: between ycdU and tRNAserX

Due to (unlucky) choice of linearization site, this island is splitted into two halves in TY-2482_chromosome.

In addition to iha and ter this island also comprises a microcin gene cluster, and a yeeSVW gene cluster.

We have termed this region the tellurite resistance- and adherence-conferring island. TarrPI-BilgeSS-2000

The considerable degree of homology between the ter genes on IncH12 plasmid R478, which originated in Serratia marcescens, and pTE53, from an E. coli clinical isolate, suggests that the pathogenicity island was acquired from a plasmid. TaylorDE-RookerM-2002

Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7. The presence of the ter gene cluster was significantly associated with the presence of eae genes. We conclude that the use of tellurite-containing media in screening for STEC will allow the detection of STEC O26, O111, O145 and non-sorbitol-fermenting O157, but most strains (in this study 74.3%) from other serotypes will be missed. OrthD-GrifK-2006

List of genes

	unigene	description	pubmed	size	sequence position
—>	B5YU69	Diguanylate cyclase (GGDEF) domain protein		1428	TY-2482_chromosome[5253101:5254528]
<—	B7LA99	IS3 element protein InsF (Transposase ORF B, IS3)	19165319	864	TY-2482_chromosome[5254575:5255438:r]
—>	Q7B9I1	Putative uncharacterized protein	12067342	273	TY-2482_chromosome[5255295:5255567]
<—	B7LFE0	IS3 element protein InsE	19165319	306	TY-2482_chromosome[5255438:5255743:r]
—>	C6UQ95	Acyl transferase	19564389	525	TY-2482_chromosome[5255801:5256325]
—>	B7LFE2	Putative uncharacterized protein ycdU	19165319	984	TY-2482_chromosome[5256325:5257308]
—>	C8UMN7	Putative integrase	19815525	1215	TY-2482_chromosome[5257678:5258892]
—>	C8TP85	Putative integrase	19815525	1233	TY-2482_chromosome[5257687:5258919]
<—	B7UP05	Predicted protein	18952797	1527	TY-2482_chromosome[5259068:5260594:r]
<—	E6B380	Prophage CP4-57 regulatory protein alpA		297	TY-2482_chromosome[5260569:5260865:r]
—>	B1LKM5	Putative uncharacterized protein	18708504	1833	TY-2482_chromosome[5260954:5262786]
—>	C8TX59	Conserved predicted protein	19815525	561	TY-2482_chromosome[5264125:5264685]
—>	C8UMP3	Putative helicase	19815525	2358	TY-2482_chromosome[5264843:5267200]
<—	B7MQ88	Putative uncharacterized protein	19165319	237	TY-2482_chromosome[5267224:5267460:r]
<—	D3GX28	Transposase	20098708	1434	TY-2482_chromosome[5267479:5268912:r]
—>	Q8FDZ2	Putative uncharacterized protein	12471157	456	TY-2482_chromosome[5268636:5269091]
<—	E5ZNV7	IS66 family element, transposase		219	TY-2482_chromosome[5268912:5269130:r]
—>	Q8FDZ2	Putative uncharacterized protein	12471157	147	TY-2482_chromosome[5269013:5269159]
<—	Q8XC14	Putative IS encoded protein encoded within prophage CP-933O	11206551	495	TY-2482_chromosome[5269155:5269649:r]
<—	C8TKW2	Putative uncharacterized protein	19815525	402	TY-2482_chromosome[5269499:5269900:r]
<—	C8UMT3	Conserved predicted protein	19815525	222	TY-2482_chromosome[5270141:5270362:r]
<—	B7LBX4	Putative Iha adhesin ( receptor)	19165319	2088	TY-2482_chromosome[5270599:5272686:r]
—>	C8TNI9	Putative glucosyl-transferase	19815525	630	TY-2482_chromosome[1:630]
<—	D3GUR0	Putative microcin esterase	20098708	1272	TY-2482_chromosome[778:2049:r]
<—	D7JUK2	Transposase		545	TY-2482_chromosome[2341:2885:r]
<—	P0CE57	Transposase insH for insertion sequence element IS5R	8041620;9097039;9097040;9278503;16738553	981	TY-2482_chromosome[2658:3638:r]
<—	D3GUQ8	Putative uncharacterized protein mchS3	20098708	432	TY-2482_chromosome[3791:4222:r]
<—	D3GUQ7	Putative uncharacterized protein mchS4	20098708	243	TY-2482_chromosome[4381:4623:r]
—>	D3GUQ6	Microcin H47 immunity protein	20098708	207	TY-2482_chromosome[5035:5241]
—>	D3GUQ5	Microcin H47	20098708	225	TY-2482_chromosome[5272:5496]
—>	D3GUQ4	Putative microcin H47 biosynthesis protein	20098708	1548	TY-2482_chromosome[5771:7318]
—>	Q83Z99	Putative acyltransferase MchD	12949180	450	TY-2482_chromosome[7347:7796]
—>	C3VUZ6	McnA	20979348	1272	TY-2482_chromosome[7952:9223]
—>	D3GUQ1	Probable microcin H47 secretion/processing ATP-binding protein (EC 3.4.22.-)	20098708	2100	TY-2482_chromosome[9219:11318]
—>	B8CJ73	Toxin secretion ATP-binding protein	18398463	2118	TY-2482_chromosome[9261:11378]
<—	Q6KDB0	McmM protein	15292145	683	TY-2482_chromosome[12320:13002:r]
<—	C8TNJ2	Putative membraneprotein	19815525	456	TY-2482_chromosome[13105:13560:r]
—>	D8BCG8	Putative uncharacterized protein		243	TY-2482_chromosome[13319:13561]
—>	A1YN25	Putative uncharacterized protein	17506902	189	TY-2482_chromosome[13625:13813]
—>	C8UMU7	Putative uncharacterized protein	19815525	507	TY-2482_chromosome[14018:14524]
—>	B7LG70	Putative uncharacterized protein	19165319	657	TY-2482_chromosome[14580:15236]
<—	A1AJ16	Putative uncharacterized protein	17293413	402	TY-2482_chromosome[14884:15285:r]
—>	B7LG74	Putative uncharacterized protein	19165319	306	TY-2482_chromosome[15260:15565]
—>	E3PN47	Putative uncharacterized protein	20802035	276	TY-2482_chromosome[15519:15794]
—>	Q6WPL3	Putative uncharacterized protein S14	14977955	642	TY-2482_chromosome[16474:17115]
—>	C8UMV2	Putative UvrD/REP helicase-like protein	19815525	2679	TY-2482_chromosome[17360:20038]
—>	B7LFS5	Immunoglobulin-binding regulator	19165319	1230	TY-2482_chromosome[20550:21779]
—>	E1U309	Transposase InsAB'	20856797	695	TY-2482_chromosome[21915:22609]
—>	Q9LA20	InsB	10594830	841	TY-2482_chromosome[22628:23468]
—>	E3Y4T4	Integrase core domain protein		928	TY-2482_chromosome[22697:23624]
<—	B1LPS0	Anaerobic C4-dicarboxylate transporter, DcuA family	18708504	1296	TY-2482_chromosome[23657:24952:r]
<—	B1EEY8	Aspartate racemase (EC 5.1.1.13)		717	TY-2482_chromosome[25021:25737:r]
—>	D8AHZ6	LysR substrate binding domain protein		942	TY-2482_chromosome[26082:27023]
<—	P59697	Transposase for insertion sequence element IS200	11677608;12644504	457	TY-2482_chromosome[27147:27603:r]
—>	Q8FEA4	Putative uncharacterized protein	12471157	237	TY-2482_chromosome[27522:27758]
<—	D8AHZ3	Conserved domain protein (Fragment)		366	TY-2482_chromosome[28012:28377:r]
<—	D3GX03	Insertion sequence IS100, ATP-binding protein	20098708	353	TY-2482_chromosome[28757:29109:r]
<—	Q8GA77	ORF B protein	12379716	385	TY-2482_chromosome[28767:29151:r]
<—	Q5I3K9	Aec56	16428402	666	TY-2482_chromosome[29017:29682:r]
<—	C1MF88	Protein kinase subdomain-containing protein		1539	TY-2482_chromosome[29773:31311:r]
<—	A7MPV8	Putative uncharacterized protein		1635	TY-2482_chromosome[31298:32932:r]
<—	Q19NM8	TerY3	16940062	1038	TY-2482_chromosome[32935:33972:r]
<—	Q19NM5	TerY1	16940062	609	TY-2482_chromosome[33388:33996:r]
<—	Q19NM7	TerY2	16940062	639	TY-2482_chromosome[34060:34698:r]
<—	P75012	Tellurium resistance protein TerX		636	TY-2482_chromosome[34698:35333:r]
<—	Q19NM5	TerY1	16940062	717	TY-2482_chromosome[35359:36075:r]
<—	P75010	Tellurium resistance protein terW	8981981	465	TY-2482_chromosome[36461:36925:r]
<—	C6UQE2	Predicted protein	19564389	1206	TY-2482_chromosome[36946:38151:r]
<—	C6UQE3	Predicted protein	19564389	2069	TY-2482_chromosome[38165:40233:r]
<—	C6UQE4	Predicted protein	19564389	771	TY-2482_chromosome[40202:40972:r]
—>	E7IZA3	Putative uncharacterized protein		321	TY-2482_chromosome[40655:40975]
<—	C6UQE5	Predicted protein	19564389	1140	TY-2482_chromosome[40968:42107:r]
<—	C8UMS5	Putative uncharacterized protein	19815525	1065	TY-2482_chromosome[42120:43184:r]
—>	C8TNH3	Putative tellurium resistance protein TerZ	19815525	579	TY-2482_chromosome[43498:44076]
—>	C8TX27	Putative tellurium resistance protein TerA	19815525	1155	TY-2482_chromosome[44079:45233]
—>	C8TX26	Putative tellurium resistance protein TerB	19815525	453	TY-2482_chromosome[45259:45711]
—>	C8TX25	Putative tellurium resistance protein TerC	19815525	1038	TY-2482_chromosome[45737:46774]
—>	C6UQF1	Putative phage inhibition, colicin resistance and tellurite resistance protein	19564389	576	TY-2482_chromosome[46826:47401]
—>	Q19NL2	TerE	16940062	778	TY-2482_chromosome[47268:48045]
—>	C8TX21	Putative tellurium resistance protein TerF	19815525	1239	TY-2482_chromosome[48479:49717]
<—	Q70Q77	Putative uncharacterized protein		534	TY-2482_chromosome[49733:50266:r]
—>	C8UCH9	Putative uncharacterized protein	19815525	423	TY-2482_chromosome[49878:50300]
—>	C8TV65	Putative uncharacterized protein	19815525	978	TY-2482_chromosome[51007:51984]
—>		ref|NC_010473|:2160347-2160550|-| [gene=isrC] [locus_tag=ECDH10B_2145]		204	TY-2482_chromosome[52093:52296]
—>	C8TPC9	Adhesin AIDA-I	19815525	783	TY-2482_chromosome[52317:53099]
—>	B7LG82	Antigen 43 (Ag43) phase-variable biofilm formation autotransporter; CP4-44 prophage	19165319	1272	TY-2482_chromosome[53074:54345]
—>	C6UQI7	Putative uncharacterized protein	19564389	2397	TY-2482_chromosome[54456:56852]
—>	C6UZH6	Putative uncharacterized protein	19564389	903	TY-2482_chromosome[56852:57754]
—>	C6UZH7	Putative uncharacterized protein	19564389	1068	TY-2482_chromosome[57754:58821]
—>	B7L946	Putative uncharacterized protein	19165319	789	TY-2482_chromosome[58850:59638]
—>	C8TX14	Putative uncharacterized protein	19815525	408	TY-2482_chromosome[59657:60064]
<—	D8EDM2	Conserved domain protein		231	TY-2482_chromosome[60067:60297:r]
—>	C8TX13	Putative uncharacterized protein	19815525	819	TY-2482_chromosome[60288:61106]
<—	Q1RA72	Putative uncharacterized protein	16585510	873	TY-2482_chromosome[60297:61169:r]
<—	Q1RA72	Putative uncharacterized protein	16585510	48	TY-2482_chromosome[61231:61278:r]
—>	Q5K5L7	Putative uncharacterized protein	11682182;12635929	540	TY-2482_chromosome[61379:61918]
<—	Q5K5L6	Putative uncharacterized protein	11682182;12635929	957	TY-2482_chromosome[61445:62401:r]
—>	D3GUT2	Putative DNA repair protein (Putative RadC-like DNA repair protein)	20098708	489	TY-2482_chromosome[61922:62410]
—>	E7H850	DNA repair RadC family protein		758	TY-2482_chromosome[61937:62694]
<—	Q6KD12	Putative uncharacterized protein	15292145	291	TY-2482_chromosome[62413:62703:r]
<—	Q8VR60	Putative uncharacterized protein	11574920	341	TY-2482_chromosome[62520:62860:r]
—>	Q707F2	YeeU protein	15385503	415	TY-2482_chromosome[62709:63123]
<—	Q8FDR3	Putative uncharacterized protein	12471157	572	TY-2482_chromosome[62725:63296:r]
—>	E9TQB2	Conserved domain protein (Fragment)		580	TY-2482_chromosome[63009:63588]
—>	Q9X5P0	Putative uncharacterized protein	10094710	486	TY-2482_chromosome[63591:64076]
—>	Q9X5P0	Putative uncharacterized protein	10094710	364	TY-2482_chromosome[63922:64285]
—>	D8A1P1	Putative uncharacterized protein (Fragment)		489	TY-2482_chromosome[64304:64792]
—>	C8TNM7	Putative uncharacterized protein	19815525	840	TY-2482_chromosome[64373:65212]
<—		ref|NC_010473|:1152233-1152320|Ser tRNA| [gene=serX] [locus_tag=ECDH10B_1104]		88	TY-2482_chromosome[65365:65452:r]

This list was extracted from Era7_EHEC_BGI_V4_Annotation.txt using rod2html.py. Sequence positions are formatted as Emboss Universal Sequence Addresses.