whether the study factor needs to be considered?

[zhangy2361]

Thank you and your team for developing the curatedMetagenomicData package. I have encountered some confusion when using this package. Firstly, whether the relative abundance table has been standardized and does not need to consider the batch of the study？ Is this the final relative abundance table?
Secondly, I downloaded a cancer data from different studies using curatedMetagenomicData, and whether the study factor needs to be considered when finding the significantly different microbiome using massLin. Or, what other analysis methods for finding the significantly different microbiome are recommended when using curatedMetagenomicData? Or, do I need to follow this tutorial (vignettes/Sex_metaanalysis_vignette.Rmd). Looking forward to your reply.