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I would like to express my deepest appreciation to dr.\ rer.\ nat. Patrick González and prof.\ dr. Marloes Groot for their guidance, feedback, and critical questions.
I am also thankful to Mengyao Zhou, and Sylvia Spies who have provided me with higher harmonic generation images of skin tissue and pediatric brain tumors.
Also thanks to the Princess Maxima Center for pediatric oncology making data sharing possible and special thanks to prof.\ dr. Pieter Wesseling for giving clinical insight.
Lastly, I'd like to acknowledge the group of prof.\ dr.\ Marloes Groot and the Biophotonics and Medical Imaging section of the VU for inspiring discussions and feedback.