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Automated minimum read length "-m" setting #143

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joshuailevy opened this issue Nov 18, 2022 · 2 comments
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Automated minimum read length "-m" setting #143

joshuailevy opened this issue Nov 18, 2022 · 2 comments
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enhancement New feature or request
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@joshuailevy
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At the moment, the default setting for the -m parameter is often too small to remove a lot of sequencing artifacts (e.g., primer dimers), but it would seem that using the actual read lengths from the dataset would be a more robust default (say, using -m 100 for a dataset with 150bp reads, possibly implemented as percentage of the expected read length?). I know a lot of users aren't aware of the importance of this parameter's value, and resulting seq outputs can potentially be impacted.

@cmaceves cmaceves self-assigned this Nov 18, 2022
@cmaceves cmaceves added the enhancement New feature or request label Nov 18, 2022
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thanks for raising this issue Josh, will keep this in mind for future releases!

@cmaceves cmaceves added this to the v1.3.3 milestone Feb 7, 2023
@cmaceves cmaceves mentioned this issue Feb 14, 2023
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addressed in PR #155

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