You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
At the moment, the default setting for the -m parameter is often too small to remove a lot of sequencing artifacts (e.g., primer dimers), but it would seem that using the actual read lengths from the dataset would be a more robust default (say, using -m 100 for a dataset with 150bp reads, possibly implemented as percentage of the expected read length?). I know a lot of users aren't aware of the importance of this parameter's value, and resulting seq outputs can potentially be impacted.
The text was updated successfully, but these errors were encountered:
At the moment, the default setting for the
-m
parameter is often too small to remove a lot of sequencing artifacts (e.g., primer dimers), but it would seem that using the actual read lengths from the dataset would be a more robust default (say, using-m 100
for a dataset with 150bp reads, possibly implemented as percentage of the expected read length?). I know a lot of users aren't aware of the importance of this parameter's value, and resulting seq outputs can potentially be impacted.The text was updated successfully, but these errors were encountered: