diff --git a/packages/react-native/ReactCommon/react/runtime/platform/ios/ReactCommon/RCTInstance.mm b/packages/react-native/ReactCommon/react/runtime/platform/ios/ReactCommon/RCTInstance.mm
index 560d5a95765c12..ffc333f06eef65 100644
--- a/packages/react-native/ReactCommon/react/runtime/platform/ios/ReactCommon/RCTInstance.mm
+++ b/packages/react-native/ReactCommon/react/runtime/platform/ios/ReactCommon/RCTInstance.mm
@@ -473,6 +473,7 @@ - (void)_loadScriptFromSource:(RCTSource *)source
     RCTExecuteOnMainQueue(^{
       onInitialBundleLoad();
     });
+    _onInitialBundleLoad = nil;
   }
 }