diff --git a/Lib/test/test_typing.py b/Lib/test/test_typing.py
index 2afac235391552..d6cd3d9bdd6a48 100644
--- a/Lib/test/test_typing.py
+++ b/Lib/test/test_typing.py
@@ -768,12 +768,18 @@ class C(Generic[*Ts]): pass
             ('generic[T, *Ts]',                        '[int]',                                          'generic[int]'),
             ('generic[T, *Ts]',                        '[int, str]',                                     'generic[int, str]'),
             ('generic[T, *Ts]',                        '[int, str, bool]',                               'generic[int, str, bool]'),
+            ('generic[list[T], *Ts]',                  '[int]',                                          'generic[list[int]]'),
+            ('generic[list[T], *Ts]',                  '[int, str]',                                     'generic[list[int], str]'),
+            ('generic[list[T], *Ts]',                  '[int, str, bool]',                               'generic[list[int], str, bool]'),
 
             ('generic[T, *Ts]',                        '[*tuple[int, ...]]',                             'TypeError'),  # Should be generic[int, *tuple[int, ...]]
 
             ('generic[*Ts, T]',                        '[int]',                                          'generic[int]'),
             ('generic[*Ts, T]',                        '[int, str]',                                     'generic[int, str]'),
-            ('generic[*Ts, T]',                          '[int, str, bool]',                             'generic[int, str, bool]'),
+            ('generic[*Ts, T]',                        '[int, str, bool]',                               'generic[int, str, bool]'),
+            ('generic[*Ts, list[T]]',                  '[int]',                                          'generic[list[int]]'),
+            ('generic[*Ts, list[T]]',                  '[int, str]',                                     'generic[int, list[str]]'),
+            ('generic[*Ts, list[T]]',                  '[int, str, bool]',                               'generic[int, str, list[bool]]'),
 
             ('generic[T, *tuple_type[int, ...]]',      '[str]',                                          'generic[str, *tuple_type[int, ...]]'),
             ('generic[T1, T2, *tuple_type[int, ...]]', '[str, bool]',                                    'generic[str, bool, *tuple_type[int, ...]]'),
diff --git a/Misc/NEWS.d/next/Core and Builtins/2022-05-30-10-22-46.gh-issue-93345.gi1A4L.rst b/Misc/NEWS.d/next/Core and Builtins/2022-05-30-10-22-46.gh-issue-93345.gi1A4L.rst
new file mode 100644
index 00000000000000..8a11f473252183
--- /dev/null
+++ b/Misc/NEWS.d/next/Core and Builtins/2022-05-30-10-22-46.gh-issue-93345.gi1A4L.rst	
@@ -0,0 +1,2 @@
+Fix a crash in substitution of a TypeVar in nested generic alias after
+TypeVarTuple.
diff --git a/Objects/genericaliasobject.c b/Objects/genericaliasobject.c
index 39fd70999ecbe5..59420816496f09 100644
--- a/Objects/genericaliasobject.c
+++ b/Objects/genericaliasobject.c
@@ -296,7 +296,7 @@ subs_tvars(PyObject *obj, PyObject *params,
             else {
                 if (iparam >= 0) {
                     if (iparam > varparam) {
-                        iparam += nargs - nsubargs;
+                        iparam += nargs - nparams;
                     }
                     arg = argitems[iparam];
                 }