diff --git a/Tests/check_fli_oob.py b/Tests/check_fli_oob.py
index ac46ff1ebc0..e0057a2c2ad 100644
--- a/Tests/check_fli_oob.py
+++ b/Tests/check_fli_oob.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python3
 from __future__ import annotations
 
 from PIL import Image
diff --git a/Tests/images/create_eps.gnuplot b/Tests/images/create_eps.gnuplot
index 4d7e2987769..57a3c8c9725 100644
--- a/Tests/images/create_eps.gnuplot
+++ b/Tests/images/create_eps.gnuplot
@@ -1,5 +1,3 @@
-#!/usr/bin/gnuplot
-
 #This is the script that was used to create our sample EPS files
 #We used the following version of the gnuplot program
 #G N U P L O T
diff --git a/Tests/oss-fuzz/fuzz_pillow.py b/Tests/oss-fuzz/fuzz_pillow.py
index e6e99d415a6..9137391b656 100644
--- a/Tests/oss-fuzz/fuzz_pillow.py
+++ b/Tests/oss-fuzz/fuzz_pillow.py
@@ -1,5 +1,3 @@
-#!/usr/bin/python3
-
 # Copyright 2020 Google LLC
 #
 # Licensed under the Apache License, Version 2.0 (the "License");
diff --git a/setup.py b/setup.py
index 1bf0bcff558..1bbd2c05cc9 100644
--- a/setup.py
+++ b/setup.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python3
 # > pyroma .
 # ------------------------------
 # Checking .