diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 14180b7c..9206974c 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -79,6 +79,24 @@ jobs:
       env:
         DOCKER_DEFAULT_PLATFORM: ${{ matrix.platform }}
 
+  validate-platforms:
+    name: Validate platforms
+    needs: build
+    runs-on: ubuntu-latest
+    steps:
+    - uses: actions/checkout@v3
+
+    - uses: docker/setup-qemu-action@v2
+
+    - uses: docker/login-action@v2
+      with:
+        registry: ghcr.io
+        username: nextstrain-bot
+        password: ${{ secrets.GH_TOKEN_NEXTSTRAIN_BOT_MANAGE_PACKAGES }}
+
+    - name: Validate final images
+      run: ./devel/validate-platforms -r ghcr.io -t ${{ needs.build.outputs.tag }}
+
   push-branch:
     if: startsWith(needs.build.outputs.tag, 'branch-') && github.event_name != 'pull_request'
     needs: build
@@ -103,7 +121,7 @@ jobs:
 
   push-build:
     if: startsWith(needs.build.outputs.tag, 'build-')
-    needs: [build, test]
+    needs: [build, test, validate-platforms]
     runs-on: ubuntu-latest
     steps:
     - uses: actions/checkout@v3
@@ -126,7 +144,7 @@ jobs:
 
   cleanup-registry:
     if: always()
-    needs: [build, test, push-branch, push-build]
+    needs: [build, test, validate-platforms, push-branch, push-build]
     runs-on: ubuntu-latest
     steps:
     - uses: actions/checkout@v3
diff --git a/devel/validate-platforms b/devel/validate-platforms
new file mode 100755
index 00000000..360c4336
--- /dev/null
+++ b/devel/validate-platforms
@@ -0,0 +1,118 @@
+#!/bin/bash
+#
+# Validate different platform builds of the final Nextstrain image.
+#
+set -euo pipefail
+
+# Set default values.
+registry=localhost:5000
+tag=latest
+
+# Read command-line arguments.
+while getopts "r:t:" opt; do
+    case "$opt" in
+        r) registry="$OPTARG";;
+        t) tag="$OPTARG";;
+        *) echo "Usage: $0 [-r <registry>] [-t <tag>]" 1>&2; exit 1;;
+    esac
+done
+
+IMAGE="$registry/nextstrain/base:$tag"
+
+main() {
+    report_names=()
+
+    version_commands=(
+        "nextclade --version"
+        "augur --version"
+        "auspice --version"
+        "pip show evofr | grep Version"
+        "datasets --version"
+        "dataformat version"
+    )
+
+    for platform in linux/amd64 linux/arm64; do
+        echo "[$platform] Pulling image..."
+        docker pull -q --platform "$platform" "$IMAGE"
+
+        echo "[$platform] Checking that the platform is expected..."
+        check-platform "$platform"
+
+        # Initialize a report file for each platform.
+        normalized_platform="$(normalize-platform "$platform")"
+        report_name="report_$normalized_platform"
+        :> "$report_name"
+        report_names+=("$report_name")
+
+        # Check software where downloaded versions are not deterministic at
+        # different points in time (e.g. if "latest" is downloaded).
+        echo "[$platform] Determining software versions..."
+        for command in "${version_commands[@]}"; do
+            report-output "$platform" "$command" >> "$report_name"
+        done
+    done
+
+    # Compare contents of the first platform's report file against others.
+
+    first_report="${report_names[0]}"
+    echo "The first report ($first_report) has the following contents:"
+    cat "$first_report"
+
+    echo "Comparing against other platforms..."
+    for report in "${report_names[@]:1}"; do
+        diff -U 1 "$first_report" "$report"
+    done
+
+    echo "Success!"
+
+    # TODO: cleanup on error
+    rm -rf "${report_names[@]}"
+}
+
+report-output() {
+    # Print a section of the report for the output of a given command.
+    local platform="$1"
+    local command="$2"
+
+    echo "Output of \"$command\":"
+    echo "$(docker-run "$platform" "$command")"
+    echo
+}
+
+normalize-platform() {
+    local platform="$1"
+
+    # Replace '/' with '_'
+    echo "${platform//\//_}"
+}
+
+check-platform() {
+    # Check that the platform is actually what we expect it to be.
+    local platform="$1"
+
+    python_platform_string="$(docker-run "$platform" "python -c \"import platform; print(platform.platform())\"")"
+
+    case "$platform" in
+        linux/amd64)
+            if [[ "$python_platform_string" != *"x86_64"* ]]; then
+                echo "Platform $platform not detected." 1>&2; exit 1
+            fi;;
+        linux/arm64)
+            if [[ "$python_platform_string" != *"aarch64"* ]]; then
+                echo "Platform $platform not detected." 1>&2; exit 1
+            fi;;
+        *)
+            echo "Platform $platform not supported." 1>&2; exit 1;;
+    esac
+}
+
+docker-run() {
+    # Run a command under the final Nextstrain image built for a specific
+    # platform.
+    local platform="$1"
+    local command="$2"
+
+    docker run --platform "$platform" "$IMAGE" bash -c "$command"
+}
+
+main