diff --git a/workflows/long-read-mngs/run.wdl b/workflows/long-read-mngs/run.wdl
index e1ffb2a1..99a2f34d 100644
--- a/workflows/long-read-mngs/run.wdl
+++ b/workflows/long-read-mngs/run.wdl
@@ -1280,7 +1280,7 @@ workflow czid_long_read_mngs {
         String library_type = "RNA"
         String guppy_basecaller_setting = "hac" # fast, hac, super
 
-        Int subsample_depth = 4000000 # should be 4x the number of reads desired
+        Int subsample_depth = 1000000 
 
         File minimap_host_db
         File minimap_human_db