diff --git a/plugins/performance-lab/includes/site-health/audit-autoloaded-options/hooks.php b/plugins/performance-lab/includes/site-health/audit-autoloaded-options/hooks.php
index 95f9036752..d1acf58e2e 100644
--- a/plugins/performance-lab/includes/site-health/audit-autoloaded-options/hooks.php
+++ b/plugins/performance-lab/includes/site-health/audit-autoloaded-options/hooks.php
@@ -129,3 +129,25 @@ function perflab_aao_extend_core_check( string $description ): string {
 	return $description . perflab_aao_get_autoloaded_options_table() . perflab_aao_get_disabled_autoloaded_options_table();
 }
 add_filter( 'site_status_autoloaded_options_limit_description', 'perflab_aao_extend_core_check' );
+
+/**
+ * Filters the list of disabled options to exclude options that are autoloaded.
+ *
+ * This filter modifies the 'option_perflab_aao_disabled_options' to ensure
+ * that autoloaded options are not included in the disabled options list.
+ *
+ * @since n.e.x.t
+ *
+ * @param string[]|mixed $disabled_options Array of disabled options.
+ * @return string[] Filtered array of disabled options excluding autoloaded options.
+ */
+function perflab_filter_option_perflab_aao_disabled_options( $disabled_options ): array {
+	$autoload_option_names = wp_list_pluck( perflab_aao_query_autoloaded_options(), 'option_name' );
+	return array_filter(
+		(array) $disabled_options,
+		static function ( $option ) use ( $autoload_option_names ): bool {
+			return ! in_array( $option, $autoload_option_names, true );
+		}
+	);
+}
+add_filter( 'option_perflab_aao_disabled_options', 'perflab_filter_option_perflab_aao_disabled_options' );
diff --git a/plugins/performance-lab/tests/includes/site-health/audit-autoloaded-options/test-audit-autoloaded-options.php b/plugins/performance-lab/tests/includes/site-health/audit-autoloaded-options/test-audit-autoloaded-options.php
index 99c69f84f8..8c5a557a15 100644
--- a/plugins/performance-lab/tests/includes/site-health/audit-autoloaded-options/test-audit-autoloaded-options.php
+++ b/plugins/performance-lab/tests/includes/site-health/audit-autoloaded-options/test-audit-autoloaded-options.php
@@ -121,12 +121,7 @@ public function test_perflab_aao_autoloaded_options_disable_revert_functionality
 		wp_set_current_user( $user_id );
 
 		// Mock wp_redirect to avoid actual redirection.
-		add_filter(
-			'wp_redirect',
-			static function () {
-				return false;
-			}
-		);
+		add_filter( 'wp_redirect', '__return_false' );
 
 		// Add an autoload option with small size length value for testing.
 		$test_option_string       = 'test';
@@ -177,14 +172,48 @@ static function () {
 		// Test that the reverted autoloaded option is displayed in the autoloaded options perflab_aao_get_autoloaded_options_table().
 		$table_html = perflab_aao_get_autoloaded_options_table();
 		$this->assertStringContainsString( self::AUTOLOADED_OPTION_KEY, $table_html );
+	}
 
-		// Remove the mock filter.
-		remove_filter(
-			'wp_redirect',
-			static function () {
-				return false;
-			}
-		);
+	/**
+	 * Test that the list of disabled options excludes options that are autoloaded.
+	 *
+	 * @covers ::perflab_filter_option_perflab_aao_disabled_options
+	 */
+	public function test_perflab_aao_autoloaded_options_auto_enable_functionality(): void {
+
+		$user_id = self::factory()->user->create( array( 'role' => 'administrator' ) );
+		wp_set_current_user( $user_id );
+
+		// Mock wp_redirect to avoid actual redirection.
+		add_filter( 'wp_redirect', '__return_false' );
+
+		// Add an autoload option with bigger size length value for testing.
+		self::set_autoloaded_option( self::WARNING_AUTOLOADED_SIZE_LIMIT_IN_BYTES );
+
+		$table_html = perflab_aao_get_autoloaded_options_table();
+		$this->assertStringContainsString( self::AUTOLOADED_OPTION_KEY, $table_html );
+
+		// Check disable autoloaded option functionality.
+		$_REQUEST['_wpnonce'] = wp_create_nonce( 'perflab_aao_update_autoload' );
+		$_GET['action']       = 'perflab_aao_update_autoload';
+		$_GET['option_name']  = self::AUTOLOADED_OPTION_KEY;
+		$_GET['autoload']     = 'false';
+
+		perflab_aao_handle_update_autoload();
+
+		// Test that the autoloaded option is not displayed in the perflab_aao_get_autoloaded_options_table() after disabling.
+		$table_html = perflab_aao_get_autoloaded_options_table();
+		$this->assertStringNotContainsString( self::AUTOLOADED_OPTION_KEY, $table_html );
+
+		// The option already exists, so update it.
+		update_option( self::AUTOLOADED_OPTION_KEY, wp_generate_password( self::WARNING_AUTOLOADED_SIZE_LIMIT_IN_BYTES ), 'yes' );
+
+		$table_html = perflab_aao_get_autoloaded_options_table();
+		$this->assertStringContainsString( self::AUTOLOADED_OPTION_KEY, $table_html );
+
+		// Test that the disabled autoloaded option is displayed in the disabled options perflab_aao_get_disabled_autoloaded_options_table().
+		$table_html = perflab_aao_get_disabled_autoloaded_options_table();
+		$this->assertStringNotContainsString( self::AUTOLOADED_OPTION_KEY, $table_html );
 	}
 
 	/**